Testing equipment 11 accessory 1 calibration 2 calorimeter 2 colorimeter 0. Mastercycler ep realplex software setup for the quantifast probe. This eppendorf cyclemanager pro software allows control of up to 30 mastercycler pro with one computer. Mastercycler nexus x2 cyclers, pcr eppendorf india. Eppendorf mastercycler ep realplex realtime pcr system. The advanced cyclemanager pro software offers a clientserver architecture, that enables one to check the status of the pcr from any computer in. Enjoy peace of mind with eppendorf performance plans and services for your mastercycler pro, mastercycler ep and mastercycler family thermal cyclers.
With less than 40 db a noise emission, the nexus is whisper quiet, while also being energy efficient. Mastercycler ep realplex is compact in size, so it can fit in virtually any lab, no matter how limited the benchspace. Temperature control is carried out with peltier elements, which enable extremely rapid changes in. Ive been using the veriti thermal cycler even for gradients tests. The mastercycler nexus x2 is eppendorfs solution for busy pcr labs.
Connection of several mastercycler ep to one control panel. The company operates in more than 30 countries and delivers an extensive portfolio of products and services. Used eppendorf mastercycler ep realplex 2 realtime pcr. Eppendorfs mastercycler ep realplex is an instrument that exceeds the demand for rapid, accurate and reliable results. Our mastercycler pro has a special lid to avoid evaporation and superior block homogeneity to achieve consistent results, despite the well being used. Mastercycler nexus 360view mastercyclernexusgradient the mastercycler nexus can accommodate 96well pcr plates, 0. The 96 well aluminium block can accomodate several standard vessels types from 0.
Control of this pcr system is easy thanks to the cycle manager pro software, that gives you a high degree of flexibility, and guides users with a easy to understand graphic interface. We enable science by offering product choice, services, process excellence and our people make it happen. I havent used any of the cyclers you suggest, but ive been using eppendorfs mastercycler ep realplex for realtime pcr and i find it extremely reliable reverse transcription done with. If you need a ultrafast pcr between 515 minutes for most 30 cycle programs machine. Mastercycler mastercycler gradient operating manual. Comprehensive yet intuitive software paired with recent advances in realtime pcr technology make the mastercycler ep realplex a critical contribution to modern science and research. Eppendorf mastercycler ep realplex instrument setup instructions for. Five different colors can help organize your workflow. This allows two pcr users in the lab to start and end their pcr reactions whenever they want. Temperature control is carried out with peltier elements, which enable extremely rapid changes in temperature of up to 3 c per second. The new mastercycler x50 is the elegant synthesis of speed, flexibility and pcr optimization for research pcr applications and standardization for routine pcr applications such as food testing etc. Mit dem mastercycler nexus x2 konnen sie zwei vollig unabhangige protokolle gleichzeitig ausfuhren.
This pcr system uses a control panel to manage reactions, and can be operated through the key pad or with use of a mouse to easily edit and store up to 700 protocols. Amplify your performance to ensure your instrument is operating consistently at peak performance, we have developed service programs and options to meet your needs. In our laboratory, we use it routinely for quantitative mrna expression studies, verification of microarray results and pathogen mycoplasma detection in eukaryotic systems. Two totally independent protocols can be run on the two blocks. Uber unseren besonders intuitiven touchscreen sind all diese vorteile stets verfugbar. A minimal number of moving parts in the optical detection module minimizes the vulnerability of the overall system and promotes consistently high sensitivity in your realtime pcr experiments. A new highly intuitive touch screen concept puts all of those benefits always at your fingertips.
The eppendorf mastercycler and mastercycler gradient are pcr devices for all molecular biological and biochemical laboratories in the. Abi 7500 applied biosystems, foster city, ca and mastercycler ep realplex eppendorf, hamburg, germany were used in shanghai. Reliance onestep multiplex supermix is designed to efficiently detect both rna and dna targets in the same reaction. Features and benefits sensitive, reproducible multiplexing of up to 5 targets get the most out of your samples while reducing costs. Find online auctions and classified ads for eppendorf products and more on the labx marketplace. Ive been using more eppendorfs ep mastercycler gradient and i will be getting. Most differences are obviously in the application software, especially in the way of data analysis and how. Whether samples are tested for both rna and dna viruses at the same time or are run with internal controls, reliance onestep multiplex supermix. The machine features, like some other mastercyler devices from eppendorf, a special 96well heating block, which enables rapid thermal cycling. Mastercycler ep realplex from eppendorf biocompare product.
Comprehensive yet intuitive software paired with recent advances in realtime pcr technology make the mastercycler ep realplex a critical contribution to modern science and resear. Hence, the time it takes to complete an assay is decreased. As our channel brand, vwr offers an integrated, seamless purchasing experience that is optimized for the way our. Sample processing solutions for laboratorydeveloped tests. Browse ads and contact the seller directly or request. Mastercycler ep realplex from eppendorf selectscience.
Mastercycler nexus cyclers, pcr eppendorf international. Shop online for a wide selection of eppendorf mastercycler pro pcr system proprietary tect technology reduces evaporation to a minimum, improving reproducibility. This can be achieved if a qpcr instrument with a temperature gradient block is available. The thermoblock, the inner side of the heated lid and micro test tubes pcr plates can. The eppendorf mastercycler pro offers ultimate flexibility for your pcr applications. The mastercycler ep realplex features the latest technology, united with good design and a quality finish. The eppendorf mastercycler ep 384 thermal cycler system uses an aluminum block, which is a great conductor of heat and will heat uniformly throughout.
Primer optimization using temperature gradient protocol. The thermoblock, the inner side of the heated lid and micro test tubes pcr. Learn more about thermal cyclers, mastercycler ep realplex. Mar 20, 2008 the eppendorf mastercycler realplex 2 is a small, robustly designed instrument for realtime pcr. Eppendorf mastercycler ep gradient thermal cycler 96 well. Mastercycler x50 cycler, pcr eppendorf deutschland. Mastercycler ep realplex realtime pcr solutions eppendorf has developed a realtime pcr system that meets the requirements of the latest quantitative pcr applications. The eppendorf mastercycler ep gradient s is a thermal cycler in 96well format. Up to 30 units of any block format combination can be controlled with one computer. Eppendorf mastercycler ep realplex instrument setup instructions for rt2 profiler pcr arrays. The mastercycler pro is optimized for high throughput capabilities.
It is a compact, easy to use and flexible platform. Reliance onestep multiplex supermix, 1,000 x 20 l rxns, 5. The mastercycler nexus can accommodate 96well pcr plates, 0. Modified slip cover light transmissive cover for optical openings in heated platen assembly, us patent pending. Mastercycler x50 cyclers, pcr eppendorf international. Configuration of the database tool is only accessible for an administrator. Effects of plate position, plate type and sealing systems on real. This is a critical software update as ip addressing issues may lead to board failure, requiring board replacement. T a of the primers by testing identical reactions containing a fixed primer concentration, across a range of annealing temperatures.
Mastercycler ep realplex realtime pcr system eppendorf. Mastercycler nexus cyclers, pcr eppendorf south pacific. The control panel is also supplied through the mains connection of the mastercycler ep. In a normal working day in our laboratory, the realplex 2 can be run four to five times, depending upon assay type. Data were analyzed using eppendorf mastercycler ep realplex 2. Cellsdirect onestep qrtpcr kit with rox, for abi 7000, 7300, 7700, and 7900ht instruments. Melting curve analysis was performed with the default settings on the abi 7500 system sds 1. The thermoblock, the inner side of the heated lid and micro test tubes pcr plates can reach temperatures. This flexibility allows pcr users to select the consumables that best fit their assay. Available realtime rtpcr cycler and kinetic pcr machines.
The mastecycler pro is unparalleled in its ability to fulfill requirements. Development and interlaboratory validation of unlabeled. Peltier elements control the temperature of the silver coated block with extremely fast heating and cooling rates. Mastercycler services from eppendorf selectscience.
The epmotion 5070s pipetting technology is based on the classic eppendorf pistonstroke pipettes. Its compatibility to a wide range of predefined consumables also allows established procedures to be maintained. We enable science by offering product choice, services, process excellence and our people make. For completion, the following features concerning operation of the mastercycler ep realplex are not accessible to restricted users. One approach to assay optimization is to determine the optimum annealing temperature t a of the primers by testing identical reactions containing a fixed primer concentration, across a range of annealing temperatures. Eppendorf 950010045 mastercycler ep gradient s thermal. Combine any mix of mastercycler pro gradient s, mastercycler pro gradient, and mastercycler pro 384 to a network of up to 30 units and control the entire network with one computer. Reliance onestep multiplex supermix is an ideal solution for multiplexed rtqpcr, especially for pathogenviral detection and high throughput screening applications.
Mastercycler nexus x2 cycler, pcr eppendorf deutschland. Cyclemanager pro software benefit from a clientserver architecture and full software functionality. The mastercycler nexus pcr cycler provides scientists with flexibility in many forms. Use the realtime pcr master mix selection tool to find optimized master. In addition, eppendorf offers a temperature validation. Eppendorf cyclemanager pro software life sciences, life. Connection of mastercycler ep realplex to a computer. The eppendorf mastercycler family offers ultimate reliability in pcr thermal cyclers. Aug 20, 2009 mastercycler ep realplex is compact in size, so it can fit in virtually any lab, no matter how limited the benchspace. Most differences are obviously in the application software, especially in the way of. The advanced cyclemanager pro software offers a clientserver architecture, that enables one to check the status of the pcr from any computer in your local network. Browse ads and contact the seller directly or request a quote for more details.
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